Comparison of two juvenile hormone radioimmunoassays.

Growth hormone determination in children using an immunofunctional assay in comparison to conventional assays.

Conventional assays for determination of growth hormone (GH) in serum measure immunoreactive molecules of a blood sample. The immunofunctional assay (IFA), on the other hand, is able to determine biologically active molecules. In our study, we evaluated GH determination in children with IFA to compare these data with clinical reliable data of conventional assay systems, since there is only insu...

Aberrant results for peptide hormone radioimmunoassays in the presence of antibodies to rabbit IgG in patients' sera.

Internal sample attenuator counting (ISAC). A new technique for separating and measuring bound and free activity in radioimmunoassays.

I describe a new method for the separation and counting of bound and free activity in radioimmunoassays. Particles containing a radiation-absorbing (attenuating) material are added to the assay. They shield the radiation from either the antibody-bound or the free radioligand. This obviates such manipulations conventionally involved in the separation and counting steps of radioimmunoassays as ce...

Comparison of five radioimmunoassays and enzyme bioassay for measurement of digoxin in blood.

Digoxin-containing sera from 86 patients were analyzed for this drug by bioassay and radioimmunoassay. Each serum was analyzed in duplicate by six procedures: inhibition of Na+-K+-dependent ATPase and five "kit" radioimmunoassays from four different commercial sources. Mean values for two of the radioimmunoassays differed significantly from those for the bioassay. One radioimmunoassay mean valu...

Two disulphide bridges are present in juvenile hormone binding protein from Galleria mellonella.

The hemolymph juvenile hormone binding protein (JHBP) from Galleria mellonella contains two disulphide bridges/molecule and no free Cys residues. An alignment of primary structures of other Lepidopteran JHBPs indicates that Cys residues, equivalent to Cys10,17,151,195 in G. mellonella JHBP, maybe involved in -S-S- bridge formation.